The development of highly sensitive and selective biosensors has become increasingly crucial in various fields, including medical diagnostics, environmental monitoring, and food safety. Chirality is a fundamental property of nature, where molecules exist as non-superimposable mirror images, playing a critical role in biological processes and the development of life. Chiroptical nanomaterial-based biosensors leverage the intrinsic chirality of many biological molecules, such as proteins, nucleic acids, and carbohydrates, to achieve remarkable selectivity in complex biological environments. These chiroptical nanomaterials are promising candidates for next-generation biosensing platforms due to their unique optical properties and exceptional sensitivity to molecular chirality. Advanced materials that exhibit strong circular dichroism (CD) and optical rotatory dispersion (ORD) responses offer unprecedented opportunities for detecting and quantifying biomolecules with high specificity and low detection limits. By combining the principles of chiral plasmonics, metamaterials, and nanostructured surfaces, our group is designing innovative sensing architectures capable of detecting minute changes in biomolecular interactions.